Odczynniki do PCR highQu

highQu dNTP sets and mixes meet all highest industry standards and allow for unrivaled performance of your PCR and other DNA synthesis, labeling or sequencing reactions. Produced under the stringent quality monitoring conditions, they guaranty reproducible results. More than 99% HPLC purity eliminates inhibitions of PCR and allows for increased yields with higher dNTP concentrations. Exceptional stability of dNTPs allows for short term ambient temperature shipments, short term room temperature s

highQu dNTP sets and mixes meet all highest industry standards and allow for unrivaled performance of your PCR and other DNA synthesis, labeling or sequencing reactions. Produced under the stringent quality monitoring conditions, they guaranty reproducible results. More than 99% HPLC purity eliminates inhibitions of PCR and allows for increased yields with higher dNTP concentrations. Exceptional stability of dNTPs allows for short term ambient temperature shipments, short term room temperature s

highQu 1Step RT PCR Kit combines the 20X RT and RNase Inhibitor mix for efficient reverse transcription and the 2X PCR Mastermix for subsequent amplification of cDNA in the same tube. RT2 Mix, 20X is a blend of the engineered MMuLV (stable at 40-55°C allowing for high yields of long transcripts) with an efficient Ribonuclease Inhibitor protecting the template RNA from RNases. The resulting cDNA is then amplified by the 1Step RT PCR Mastermix, 2X. The PCR mastermix contains our proprietary Hot St

highQu 1Step RT qPCR master mixes in combination with a blend of thermostable and extremely active Reverse Transcriptase & advanced RNase Inhibitor (RT Mix) allow for a single step one tube RT qPCR. qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT/GC rich templates and guaranty rapid extension with early Ct va

Our improved 2X 1Step RT qPCR Probe Kits provide highly sensitive target RNA detection when working with a wide variety of samples. The optimized 1Step RT qPCR Pro Mix in combination with a blend of thermostable Reverse Transcriptase and an advanced RNase Inhibitor (RT-Pro Mix) allows for a single step, one tube RT qPCR with great results even in multiplex reactions. The novel RT-Pro Mix ensures safe and efficient RNA template conversion into a single-stranded cDNA. Pure RNA samples, as well as

Our improved 2X 1Step RT qPCR Probe Kits provide highly sensitive target RNA detection when working with a wide variety of samples. The optimized 1Step RT qPCR Pro Mix in combination with a blend of thermostable Reverse Transcriptase and an advanced RNase Inhibitor (RT-Pro Mix) allows for a single step, one tube RT qPCR with great results even in multiplex reactions. The novel RT-Pro Mix ensures safe and efficient RNA template conversion into a single-stranded cDNA. Pure RNA samples, as well as

4X 1Step RT qPCR Probe Kits are designed for sensitive detection of specific RNAs, including virus RNA, in diluted high-volume samples. They combine a robust 4X qPCR mix (with Hot Start PCR Polymerase) with a 20X blend of thermostable and extremely active Reverse Transcriptase & advanced RNase Inhibitor (RT4 Mix). This formulation allows for a high sample input volume with a reliable outcome of a single step RT qPCR when working with low copy number samples below 5 copies per reaction. 4X 1Step

4X 1Step RT qPCR Probe Kits are designed for sensitive detection of specific RNAs, including virus RNA, in diluted high-volume samples. They combine a robust 4X qPCR mix (with Hot Start PCR Polymerase) with a 20X blend of thermostable and extremely active Reverse Transcriptase & advanced RNase Inhibitor (RT4 Mix). This formulation allows for a high sample input volume with a reliable outcome of a single step RT qPCR when working with low copy number samples below 5 copies per reaction. 4X 1Step

4X 1Step RT qPCR Probe Kits are designed for sensitive detection of specific RNAs, including virus RNA, in diluted high-volume samples. They combine a robust 4X qPCR mix (with Hot Start PCR Polymerase) with a 20X blend of thermostable and extremely active Reverse Transcriptase & advanced RNase Inhibitor (RT4 Mix). This formulation allows for a high sample input volume with a reliable outcome of a single step RT qPCR when working with low copy number samples below 5 copies per reaction. 4X 1Step

U nlock PCR Multiplexing: Robust Hot Start Taq DNA Polymerase for superior PCR resul ts highQu ALLin™ Hot Start Taq Polymerase is the superior sensitive hot-start DNA polymerase. The enzyme activity at room temperature is blocked by a small molecular inhibitor. Enzyme becomes active only after heating what allows for highly specific and extremely sensitive amplification, no primer dimer formation occurs and no background appears. In combination with the optimized ALLin™ buffer enzyme provides hi

highQu ALLin™ Hot Start Taq DNA Polymerase is the superior sensitive enzyme. The activity at room temperature is blocked by small molecular inhibitor. Enzyme becomes active only after heating what allows for highly specific and extremely sensitive amplification, no primer dimer formation and no background. In combination with the optimized ALLin™ buffer enzyme provides higher success rates in demanding PCR applications like amplification of crude, complex or longer templates and fast cycling. AL

ALLin™ Isothermal 1Step RNA Amplification Kit enables detection of < 5 target RNA molecules in a short time of 30 minutes without the use of PCR cycler. An RT5 Mix, the blend of a thermostable reverse transcriptase and highly efficient RNase Inhibitor is included in the kit to initially synthesize cDNA from RNA templates. The cDNA is amplified by Bst polymerase. Kit includes a 2X master mix with optimized high-performance buffer, dNTPs and a recombinant Bst Polymerase large fragment having stron

ALLin™ Isothermal DNA Amplification Kit enables detection of as little as 5 target DNA molecules in a short time of 20 minutes even without the use of a thermal cycler. The Kit includes a 2X master mix with optimized high-performance buffer, dNTPs and a recombinant Bst Polymerase large fragment having strong 5’ - 3’ strand displacement activity and efficient 5’-3’ polymerase activity working at 55-70°C. The Polymerase has neither 5’ - 3’ nor 3’ - 5’ exonuclease activity and retains only minor re

Derived from our HiFi polymerase, the highQu ALLin™ Mega HiFi DNA Polymerase provides much lower error rate PCR with a 100 higher fidelity compared to Taq. The ALLin™ Mega HiFi DNA Polymerase is engineered to be much faster and to generate a higher yield of long PCR products up to 20 kb from complex GC-rich templates. Therefore the ALLin™ Mega HiFi DNA Polymerase is an excellent choice for longer and very complex PCR applications where the highest fidelity is demanded. It is an enzyme of choice

Derived from our HiFi polymerase, the highQu ALLin™ Mega HiFi DNA Polymerase provides much lower error rate PCR with a 100 higher fidelity compared to Taq. The ALLin™ Mega HiFi DNA Polymerase is engineered to be much faster and to generate a higher yield of long PCR products up to 20 kb from complex GC-rich templates. Therefore the ALLin™ Mega HiFi DNA Polymerase is an excellent choice for longer and very complex PCR applications where the highest fidelity is demanded. It is an enzyme of choice

Derived from our HiFi polymerase, the highQu ALLin™ Mega HiFi DNA Polymerase provides much lower error rate PCR with a 100 higher fidelity compared to Taq. The ALLin™ Mega HiFi DNA Polymerase is engineered to be much faster and to generate a higher yield of long PCR products up to 20 kb from complex GC-rich templates. Therefore the ALLin™ Mega HiFi DNA Polymerase is an excellent choice for longer and very complex PCR applications where the highest fidelity is demanded. It is an enzyme of choice

highQu ALLin™ Mega HS HiFi DNA Polymerase provides much lower error rate PCR with a 100 higher fidelity compared to Taq. Compared to Mega HiFi, this hot start enzyme version allows for even higher sensitivity and specificity of PCR as well as for a room temperature reaction setup, and is excellent choice for multiplex reactions. The ALLin™ Mega HS HiFi DNA Polymerase is engineered to be much faster and to generate higher yield of long PCR products up to 20 kb from complex GC-rich templates. Ther

highQu ALLin™ Mega HS HiFi DNA Polymerase provides much lower error rate PCR with a 100 higher fidelity compared to Taq. Compared to Mega HiFi, this hot start enzyme version allows for even higher sensitivity and specificity of PCR as well as for a room temperature reaction setup, and is excellent choice for multiplex reactions. The ALLin™ Mega HS HiFi DNA Polymerase is engineered to be much faster and to generate higher yield of long PCR products up to 20 kb from complex GC-rich templates. Ther

highQu ALLin™ Mega HS HiFi DNA Polymerase provides much lower error rate PCR with a 100 higher fidelity compared to Taq. Compared to Mega HiFi, this hot start enzyme version allows for even higher sensitivity and specificity of PCR as well as for a room temperature reaction setup, and is excellent choice for multiplex reactions. The ALLin™ Mega HS HiFi DNA Polymerase is engineered to be much faster and to generate higher yield of long PCR products up to 20 kb from complex GC-rich templates. Ther

highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (a number of correct nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. The c