highQu® SampleIN™ Lysis Set for PCR/qPCR - fast sample lysis to release the PCR-ready DNA

Opis towaru

SampleIN™ Lysis Set for PCR/qPCR is a combination of a lysis buffer and protease-containing buffer allowing for a fast lysis of different sample material to release the DNA in a short time.

SampleIN™ Lysis Set for PCR/qPCR is a part of our SampleIN™ Direct PCR Kit. SampleIN™ Direct PCR Kit includes two modules: Two Lysis/Protease Buffers (Lysis Set for PCR/qPCR) and Hot Start PCR Master Mix.

The quickly lysed samples contain enough DNA for PCR and qPCR/RT-qPCR applications, eliminating the need of tedious template purification. The set is used to prepare templates for direct PCR or qPCR from mouse tail or ear, mammalian tissues, hair follicle, buccal swabs and blood. It can also be used with some plant materials, if these are not considered as very oily, hard or difficult to lyse. 

Rapid 15 min DNA extraction using DPK Lysis and Protease Buffers in a single tube generates crude PCR template extract which can be further amplified using inhibitor resistant, robust PCR or qPCR mixes such as ALLin™ HS Red Taq Mastermix, or SampleIN™ qPCR Probe Mixes.

Applications

• Fast crude sample preparation for direct PCR/qPCR
• Template preparation for direct PCR/qPCR from mouse tail or ear, mammalian tissues (including FFPE), hair follicle, buccal swabs, and blood (including EDTA or FTA samples)

Benefits

• PCR-ready crude samples in 15 minutes
• Single-tube fast and efficient DNA release
• High yield PCR and efficient qPCR without tedious DNA extractions
• No material losses, minimized pipetting and hands on time