highQu® ALLin™ Hot Start Taq Mastermix
HSM0201
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highQu ALLin™ Hot Start Taq DNA Polymerase is the superior sensitive enzyme The activity at room temperature is blocked by small molecular inhibitor. Enzyme becomes active only after heating what allows for highly specific and extremely sensitive amplification, no primer dimer formation and no background.
In combination with the optimized ALLin™ buffer enzyme provides higher success rates in demanding PCR applications like amplification of complex or longer templates and fast cycling.
ALLin™ Hot Start Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors.
The convenience of ALLin™ Hot Start Taq DNA Polymerase is maximized by the use of 2X Mastermix providing the additional advantage of reduced pipetting and minimized errors. The mastermix is even supplied with PCR Water, and the only thing to add is the template with primers.
Applications
- Hot-start PCR up to 6 kb, Low copy target detection.
- PCR of complex (GC rich) templates.
- Fast PCR, multiplexing, TA cloning.
Benefits
- Higher yields, no background in standard or fast PCR.
- Success on longer (6 kb) or GC rich templates, in crude sample PCR.
- ALLin™ mastermix supplied with PCR Water.