highQu® ALLin™ Bst DNA Polymerase

Opis towaru

The ALLin™ Bst DNA Polymerase is a recombinant protein, representing a large fragment of the B. stearothermophilus DNA Polymerase expressed in E. coli cells. This robust polymerase with a strong strand displacement activity and high temperature tolerance ensures high amplification yield at constant temperature when working with impure or low-copy number targets as well as with complex templates. The Allin™ Bst Buffer includes optimal concentrations of magnesium and dNTPs, what minimizes pipetting steps. This ALLin™ Bst DNA Polymerase-buffer system together with a supplied enhancer, detects <5 DNA targets in a short time without the use of a thermocycler.

This glycerol-free Bst DNA Polymerase is also an excellent tool for development of dry format lyophilized kits for pathogen detection using isothermal amplification techniques.

For more convenience, we recommend our kit formulations:

ALLin™ Isothermal DNA Amplification Kit and ALLin™ Isothermal 1Step RNA Amplification Kit, both including a master mix with optimized high-performance buffer.

Technical characteristics of Bst DNA Polymerase large fragment:

• Strong 5’ - 3’ strand displacement activity
• 5’-3’ polymerase activity
• No 5’ - 3’ exonuclease activity
• No 3’ - 5’ exonuclease (proofreading) activity
• Minor reverse transcriptase activity (for RNA, use ALLin™ Isothermal 1Step RNA Amplification Kit (#IRK0101) which includes RTase)
• Optimal amplification temperature is 65°C.
• Working temperature range is 55-70°C.
• Optimal reaction time is 20 minutes, if needed, the reaction can be performed 30 to 60 minutes.
• The enzyme is inactivated in 10 minutes at 80°C.

The use of this product in certain applications may be covered by patents. The user has to analyse all applicable Limited Use Label Licenses and may need licensing for certain cases.

Applications

• Isothermal DNA amplification at elevated temperature
• Real-time detection of DNA amplification
• LAMP - loop-mediated isothermal amplification
• WGA - whole genome amplification
• RAM - ramification & RPA - recombinase polymerase amplification 

Benefits

• Glycerol-free formulation of robust Bst DNA Polymerase, supplied with all-included buffer with dNTPs and proprietary reaction enhancer
• Active at high temperatures in a range of 55-70°C 
• Fast DNA amplification at constant temperature
• Ideal for complex templates and crude samples
• Ensures <5 molecules LOD (limit of detection)