High-quality 25 mM dNTP Mix: pure dNTPs for the most demanding and sensitive PCR applications

Opis towaru

highQu dNTP sets and mixes meet all highest industry standards and allow for unrivaled performance of your PCR and other DNA synthesis, labeling or sequencing reactions. Produced under the stringent quality monitoring conditions, they guaranty reproducible results. More than 99% HPLC purity eliminates inhibitions of PCR and allows for increased yields with higher dNTP concentrations. Exceptional stability of dNTPs allows for short term ambient temperature shipments, short term room temperature storage or long PCR of more than 30 kb targets, as well as long amplifications exceeding 40 cycles.

dNTPs mixes (deoxynucleotide triphosphates) are essential components in PCR reactions

as they provide the building blocks for DNA synthesis. The dNTPs are sold in sets of separate dNTPs

or in ready-to use mixes of 10 mM or 25 mM concentration. The quality of dNTPs

used in PCR can significantly impact the success of the PCR results.
Good dNTPs are free from contaminants that can interfere with DNA amplification,

inhibit polymerase and lead to poor PCR results. Accurate and consistent dNTP concentrations

are crucial for efficient and reliable DNA synthesis during PCR, and the dNTP concentration shall correlate

with the concentration of other components, such as for example, magnesium chloride. dNTP mixes

and sets are formulated to provide balanced concentrations of dNTPs, because having equal concentration

of all four dNTPs is crucial for a smooth DNA synthesis process.

High quality dNTPs are stable and remain chemically intact over multiple freeze-thaw cycles

and extended storage periods. Analytical methods such as HPLC (high-performance liquid chromatography)

in quality testing workflows allow to control and guaranty the quality of the dNTP mixes for a long period

of up to two-three years.

Applications

SARS-CoV-2 detection by RT qPCR, COVID-19 assays.

Standard PCR, long and high-fidelity PCR.

cDNA synthesis, RT-PCR, qPCR.

Sequencing.

Benefits

Highest quality, >99% HPLC pure dNTPs.

Free of PCR inhibitors.

Highly stable.