highQu® Taq DNA Polymerase
Product Description
highQu Taq DNA Polymerase is the classical enzyme for routine PCR applications providing high amplification yields of 3-5 kb targets under various conditions. Taq DNA Polymerase is purified from a recombinant E. coli strain carrying the Taq DNA polymerase gene.
The most widely used PCR enzyme is the DNA polymerase from Thermus aquaticus. Taq DNA polymerase amplifies template DNA by adding nucleotides to the 3' end of a DNA strand in a template-dependent manner where short single-stranded primers that anneal to the template provide a starting point for polymerization.
The enzyme has a 5' - 3' polymerase activity and lacks 3' - 5' exonuclease or so-called proofreading activity. For optimal performance, Taq DNA polymerase requires not only template DNA and reverse and forward primers, but also such essential PCR reaction components as deoxyribonucleotide triphosphates (dNTPs), and magnesium chloride. Optimized buffers commonly supplied with commercial Taq Polymerase enzymes contain certain salt concentration and have balanced pH value.
Taq DNA polymerase has several other advantageous properties over other enzymes used for the PCR. It is known for a high stability and resistance to PCR inhibitors. The inhibitor resistance allows performing direct PCR reactions from crude non-purified templates, such as colony PCR. At highQu, Taq DNA Polymerase is either available as a stand-alone enzyme supplied with a ALLin™ Buffer, or as a convenient 2X concentrated master mix formulation.
Applications
- Routine PCR up to 5 kb under common and fast conditions.
- RT-PCR, colony PCR,TA cloning.
- Library construction, genotyping, screening.
Benefits
- High yields in routine PCR and under fast cycling conditions
- Robust performance under variety of conditions.
- Excellent performance on complex (GC rich) templates.