SampleIN™ Direct PCR Kit
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Manufacturer
Product Description
SampleIN™ Direct PCR Kit is a premium tool for a fast direct PCR eliminating the need of tedious template purification.
The kit is excellent for direct PCR from mouse tail or ear, mammalian tissues, hair follicle, buccal swabs and blood.
Rapid 15 min DNA extraction using DPK Lysis and Protease Buffers in a single tube generates PCR template extract which is further amplified under fast cycling conditions with a hot-start Taq master mix that includes red dye for direct gel loading.
In a 2% agarose TAE gel the red dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments.
Applications
Direct PCR without template purification.
Mouse genotyping and knockout analysis.
Direct PCR from blood, mouse tail or ear, mammalian tissues (including FFPE).
Direct PCR from hair follicle, buccal swabs, plant samples.
Benefits
Ready-to load PCR in 50 minutes without template purification.
Single-tube 15 min DNA extraction combined with fast hot-start PCR.
Red dye in the PCR master mix for direct gel loading.
High yields under standard or fast cycling conditions.
Success with GC/AT rich templates.