Reagents for electrophoresis

BIS-TRIS Propane (1,3-Bis[tris(hydroxymethyl)methylamino]propane) CAS number: [64431-96-5] C11H26N2O6 MW: 282.3 Purity: ≥ 98% pKa: 6.8 ± 0.1 Useful pH range: 6.3 - 9.5 Solubility(1M water): clear/colorless

BIS-TRIS, Free Base [Bis (2-Hydroxyethyl)amino-tris(hydroxymethyl)methane] Important buffer for protein and nucleic acid systems. CAS number: [6976-37-0] Purity: ≥ 98% C8H19NO5 MW: 209.2 pKa (25 °C): 6.35 - 6.65 Useful pH range: 5.8 - 7.2

BIS-TRIS, Free Base Molecular Biology Grade, Purity: ≥99% Bis (2-Hydroxyethyl)amino-tris(hydroxymethyl)methane]. Important buffer for protein and nucleic acid systems. Suitable for electrophoresis and cell culture. CAS number: [6976-37-0] C8H19NO5 MW: 209.2 pKa (25 °C): 6.35 - 6.65 Useful pH range: 5.8 - 7.2 Solubility (1M): clear/colorless pH, 1M water: 9.0 - 11.0 A280, 1M water: < 0.40 Heavy metals (as Pb): > 0.0005%

BRILLIANT GREEN, Certifiable Grade BRG001 CAS number: [633-03-4] Malachite Green G C27H34N2O4S MW: 482.6 Dye content: ≥ 85%

CAPS, Biotechnology Grade [3-(Cyclohexamino)-1-propanesulfonic acid] CAS number: [1135-40-6] C9H19NO3S MW: 221.3 pKa (25 °C): 10.4 Useful pH range: 9.7 - 11.1 Purity: > 99% pH (1% water): 4.9 - 6.0

CETYLDIMETHYLETHYL AMMONIUM BROMIDE CAS number: [124-03-8] C20H44NBr MW: 378.5 Purity: ≥ 99.0%

Chaps is a non-denaturing zwitterionic detergent used for solubilizing membrane proteins. Can easily be removed by dialysis or gelfiltration. 4 °C CAS number: [75621-03-3] C32H58N2O7S MW: 614.89 Purity: ≥ 98.0% pH (10%, H2O): 5.0 - 7.0 Conductivity (1M, H2O): < 100µmhos Solubility (1% water): clear/colorless Absorbance (1% water): A260/<0.05 A280/<0.05

CHES, Biotechnology Grade CHE702 CAS number: [103-47-9] [2-(N-Cyclohexylamino)ethanesulfonic acid] Useful pH: 8.6 - 10.0 pKa (20 °C): 9.55 C8H17NO3S MW: 207.3 Purity: ≥ 99.0%

highQu ready to use Prestained Protein Ladders are mixtures of highly recombinant proteins with coupled chromophores providing sharp protein bands and bright colors on denaturing polyacrylamide gels. The high ladder purity allows for exceptional stability and room short term temperature storage. Ladders are ready to be directly loaded on gels without any preparation or heating. They provide sharp bands for approximate protein sizing and allow for monitoring of electrophoresis process and Western

highQu ready to use Prestained Protein Ladders are mixtures of highly recombinant proteins with coupled chromophores providing sharp protein bands and bright colors on denaturing polyacrylamide gels. The high ladder purity allows for exceptional stability and room short term temperature storage. Ladders are ready to be directly loaded on gels without any preparation or heating. They provide sharp bands for approximate protein sizing and allow for monitoring of electrophoresis process and Western

CRESOL RED CRE350 CAS number: [1733-12-6] (O-Cresolsulfonphthalein) C21H17O5S MW: 382.4 Dye content: approx. 95%

CRESOL RED, Sodium salt CRE300 CAS number: [62625-29-0] (O-Cresolsulfonphthalein) C21H17O5SNa MW: 404.4 Dye content: approx. 95% Solubility: water

DEOXY-BIGCHAP DBG232 CAS number: [86303-23-3] N,N-Bis[3-D-Glconamidopropyl]-deoxycholamide C42H75N3O15 MW: 862.10 Purity: > 99%

DEOXYCHOLIC ACID , Sodium Salt CAS number: [302-95-4] C24H39O4Na MW: 414.60 Purity: ≥ 99.0% Heavy Metals: < 0.005% Solubility (10% water): clear

HEPES, 1M Sterile Solution HEP003 pH: 7.2 - 7.4 Nuclease activity: none detected

HEPES, Biotechnology Grade HEP001 CAS number: [7365-45-9] C8H18N2O4S MW: 238.31 Purity: ≥ 99.5% pKa (20 °C): 7.5 pH Range: 6.8 - 8.2 Heavy Metals: < 0.001% DNase, RNase, Protease activity: none detected

HEPES, Sodium Salt, Biotechnology Grade HEP002 CAS number: [75277-39-3] C8H17N2O4SNa MW: 260.3 Purity: > 99.5% Solubility (5% water): 7.55 pH (1% water): 9.5 - 11.0 Heavy Metals: < 10 ppm DNase, RNase, Protease activity: none detected

NONIDET® P-40 (SUBSTITUTE), Reagent Grade (OCTYL 1 PHENOXYPOLYETHOXYETHANOL) Chemically identical to Nonidet Density: 1.06 Color: pale yellow NONIDET® is a registered Trademark of Shell International Petroleum Co. It is no longer commercially available. IGEPAL CA 630 is a registered Trademark of Stepan Canada Inc.