Proteinase K MBG Solution, 20 mg/ml
Product Description
Proteinase K (Molecular Biology Grade Solution) is a serine peptidase with a very high specific activity
and a broad spectrum of protein digestion possibilities.
The solution is designed to be used for protein degradation (up to tetrapeptides) during the cell lysis
and RNA/DNA extraction procedures under hush reaction conditions such as the higher temperatures
and the presence of detergents. The enzyme efficiently degrades DNases and RNases during nucleic acid isolation process.
The high purity of the Proteinase K and controlled absence of both DNAse and RNase contamination ensures the integrity of nucleic acids.
The 100% enzyme activity (when stored at -20°C) is guaranteed for at least two years after production.
However, the experiments proved that the proteinase remains close to 90% active even when stored at +37°C for 18 months.
The enzyme is supplied as a 20 mg/ml concentrated solution with an average specific activity of more than 800 u/ml.
Active in all common buffers used for cell lysis and RNA/DNA extraction, in a presence of urea,
SDS and guanidinium salts
Stable at high temperature of up to 56°C
Can be inactivated by heating at 65oC for 20 minutes or at 75oC for 10 minutes
Active in a pH range of 4–12 with an optimum pH 7.5–8.0
The Proteinase K gene from album expressed in yeast host
The quality limit of allowed host DNA presence is ≤ 0.25 pg/U measured by qPCR
what is about a half when compared to other suppliers.
Unit Definition
Folin & Ciocalteu’s method - One unit is required to hydrolyze urea-denaturated hemoglobin producing color equivalent
of 1 μmol tyrosine in 1 minute at 37°C and pH 7.5, 1 U = 1 m Anson U.
Applications
Protein hydrolysis
DNAse and RNase decontamination
Protein degradation during the procedures of:
Tissue/Cell lysis
DNA extraction
Benefits
Recombinant broad spectrum, non-specific protease
Ready-to-use solution with a very high specific activity
Economical and stable for weeks at ambient temperature
Robust, works well at a wide variety of reaction conditions: up to 56oC and in a presence of urea, SDS and guanidinium salts
Safe – DNase and RNase free to ensure integrity of isolated NA