highQu® SampleIN™ 1Step RT qPCR Probe ROX L Mix, 4X - direct one step RT qPCR with inhibitor tolerant master mixes.

Product Description

SampleIN™ 1Step RT qPCR Probe ROX L Mix has been specifically designed for use with crude lysates and impure templates. This 4X concentrated single-tube RT qPCR master mix with the Hot Start Taq DNA Polymerase, Reverse Transcriptase, RNase inhibitor, dNTPs, magnesium and optimized buffer delivers an exceptional PCR inhibitor tolerance in direct one-step qPCR applications. It includes PCR enhancers and stabilizers and is formulated to provide the robust performance with such common sample materials like unpurified saliva or fresh blood. The mix tolerates a range of common chemicals present in purified DNA templates such as guanidine, alcohols, SDS and similar, as well as common blood, urine and environmental natural sample-compounds known to inhibit PCR such as hemoglobin, immunoglobulins, heparin, urea, polyphenols, cellulose, humic and tannic acids and chlorophyl.

The mix can be used with all probe types and with a variety of sample amounts from a very low-copy number targets in diluted samples to abundant templates.

The air dryabale version is available for production of room temperature stable kits: AirDry SampleIN™ 1Step RT qPCR Probe Mix, 4X. It has been specifically designed for use with unpurified sample specimens or crude lysates as templates. Inquire for OEM pricing now.

This 1Step RT qPCR Probe Mix contains all compounds required for robust single-tube RT qPCR reaction, and the only components to add are template, primers and probes. The high concentration of the master mix enables the use of maximal template volume as well as multiple probes and primers. The Mix is supplied with PCR Water.

SampleIN™ 1Step RT qPCR Probe Mix has been successfully tested for the use with fresh blood and urine samples; for more consistent results, it is always recommended to purify the template, or at least to perform fast lysis using highQu SampleIN™ Lysis Set for PCR/qPCR.

For work with crude or inhibitor-rich DNA templates, we offer a SampleIN™ Direct qPCR Probe Mixes.

SampleIN™ 1Step RT qPCR Probe ROX L Mix  includes ROX at low concentration, the version without ROX is available as SampleIN™ 1Step RT qPCR Probe Mix. If high ROX concentration or more ROX flexibility is required, ROX for qPCR Mixes, 50 μM can be obtained separately and added directly into the qPCR mix.

Tolerates common inhibitors, such as:

• 5-7% crude saliva and crude blood in the reaction
• Chemicals left after NA extractions (guanidine, alcohols, SDS) 
• Blood compounds (hematin, hemoglobin, hemin, immunoglobulins)
• Saliva and urine compounds (urea)
• Plant, soil samples (chlorophyl, humic, tannic acids, quercetin cellulose) 

Applications

• One Step RT qPCR using crude lysed or even unpurified samples
• qPCR assays based on specific probes: including TaqMan®, Molecular Beacons, Scorpions
• Quantification of RNA, viral RNA, total RNA, gDNA, cDNA, viral DNA, low copy number genes, gene expression analysis

Benefits

• Exceptional PCR-inhibitor tolerance in One-Step RT qPCR single-tube applications
• The unique composition reduces effects of common PCR inhibitors found in clinical, environmental samples, food matrices, animal, and plant materials
• Concentrated master mix containing Hot Start Tq Polymerase, Reverse Transcriptase and RNase Inhibitor for the use with maximum template volume
• Amplifies both DNA and RNA templates, great for multiplexing, excellent performance on GC-rich templates