highQu® ALLin™ HiFi DNA Polymerase
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Manufacturer
Product Description
highQu ALLin™ HiFi DNA Polymerase is the outperforming high-fidelity enzyme derived from Pfu polymerase by introducing several point mutations. The robust engineered enzyme in combination with the optimized ALLin™ buffer provides higher fidelity (50X higher than Taq DNA Polymerase), better performance, increased success in demanding applications like amplification of complex or longer templates, crude samples and fast cycling.
ALLin™ HiFi DNA Polymerase produces blunt-ended products suitable for ligating into blunt vectors.
Applications
High-fidelity PCR up to 10 kb.
Complex (GC rich) template or crude sample PCR.
Fast PCR.
Blunt cloning.
Benefits
50 x higher fidelity than classic Taq.
High sensitivity and yields under standard and fast cycling.
Increased success in long (10 kb) or GC rich PCR.
5X ALLin™ Buffer contains optimal Mg2+ and dNTPs.