EfiiJust™
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Manufacturer
Product Description
Paramagnetic bead-based post PCR clean-up reagent designed for efficient DNA purification
and consistent DNA fragment size selection for NGS. HighPrep PCR is adaptable to most liquid handling stations
and used for applications such as sequencing, NGS, microarrays, PCR, and enzymatic reactions.
APPLICATIONS
Post-PCR and post-enzymatic reaction clean-up used for/during:
NGS library preparation
DNA size selection for NGS
Microarrays
PCR
Restriction enzyme digestions, adapter ligations
Cloning
BENEFITS
Rapid and reliable post-PCR and DNA clean-up reagent
Achieve uniform and consistent DNA fragments
High recovery of amplicons >100bp
Uniform fragments size distribution
Adaptable to high throughput liquid handling workstations
Main functions | Purifies DNA and amplicons for next generation sequencing and other applications. |
Starting material (Sample matrix) | DNA, PCR products, cDNA, gDNA |
Starting amount | Scalable |
RNA recovery | >70% recovery |
Process method | Manual or automation |
Instrument | Adaptable to most nucleic acid purification instruments but a ready-made script for Kingfisher Flex is available upon request |
Purification method | Magnetic bead-based technology |
RNA Purity | DNA eluted is ready for downstream processes such as real time quantitative PCR (qPCR), next generation sequencing (NGS), cloning, restriction digestions, microarrays, etc. |
Elution Volume | 15 µl or above |