2X 1Step RT qPCR Probe Kit
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Manufacturer
Product Description
Our improved 2X 1Step RT qPCR Probe Kits provide highly sensitive target RNA detection when working with a wide variety of samples. The optimized 1Step RT qPCR Pro Mix in combination with a blend of thermostable Reverse Transcriptase and an advanced RNase Inhibitor (RT-Pro Mix) allows for a single step, one tube RT qPCR with great results even in multiplex reactions.
The novel RT-Pro Mix ensures safe and efficient RNA template conversion into a single-stranded cDNA. Pure RNA samples, as well as lysed crude samples can serve as templates for one-step RT qPCR. Though the kit was not specifically designed for crude sample qPCR, it has a potential to work well for this application.
The robust 1Step RT qPCR Pro Mix is a 2X concentrated qPCR master mix which includes dNTPs, buffer, and the hot start Taq DNA Polymerase. The Hot Start function ensures the highest sensitivity and specificity under both standard and fast qPCR cycling conditions.
The kits provide excellent results on both AT and GC-rich templates and show early Ct values with minimum or no optimization.
Our kits include PCR Water to guaranty the best performance. To suit the broad instrument range the 2X 1Step RT qPCR Probe Kits are available in three versions – without ROX, with low or high ROX concentration.
Applications
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RT qPCR assays based on specific probes: including TaqMan®, Molecular Beacons, Scorpions™ Probes
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Quantification of any RNA template (mRNA, total RNA, viral RNA), low copy number genes
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Detection of RNA viruses
Benefits
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Reverse transcription and qPCR in one tube
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Increased sensitivity, highest specificity
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Efficient cDNA synthesis from various template amounts
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Universal - both standard and fast cycling, GC/AT rich templates
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Excellent performance in multiplex assays