DNAzol® ES, Extra Strength

Product Description

DNAzol^® ES, Extra Strength

Is an extra strength DNAzol^® reagent specifically formulated for the isolation of genomic DNA from plants.

The patented procedure (U.S. patent no. 5,945,515) is based on the use of a novel guanidine-detergent

lysing solution that allows the selective precipitation of DNA from the lysate [1].

The DNAzol^® ES protocol is fast and permits efficient isolation of genomic DNA from a variety of plant tissues [2, 3].
In the DNAzol^® ES procedure, plant samples are pulverized in liquid nitrogen and genomic DNA is solubilized in DNAzol^® ES.

After a brief incubation, the samples are extracted with chloroform and centrifuged. The resulting aqueous phase is collected
and DNA is precipitated with ethanol. The pellet is resuspended and washed with a DNAzol^® ES - ethanol wash solution followed
by an ethanol wash and DNA solubilization.

The entire procedure can be completed in about 90 minutes and the isolated DNA can
be used for Southern analysis, dot blot hybridization, molecular cloning,

PCR and other molecular biology and biotechnology applications.