Corning® Cell Counter
Product Description
Time-saving Technology using the Power of the Cloud
For years, the choice between manual and automated cell counting has been a difficult one. Manual cell counting can be accurate, but time-consuming and user-dependent. Automated cell counting is much faster and less user-dependent, but the cost of disposable counting slides can be an issue. A tough choice, but now there is an affordable solution. The Corning® Cell Counter is the first automated cell counter that combines the best of both worlds.
It is:
Evolving – periodic, seamless upgrades, added features, and improved functionality.
Accurate – thanks to its cloud-based machine learning algorithm.
Improved resolution – ability to accurately count as small as 4 µm (e.g., PBMC).
Low-cost – works with common reusable glass hemocytometers. No consumables required.
Fast – thanks to its online image processing.
Evolving
Cloud-based processing allows the cell counter to add features and functionality based on user needs, unlike static non-cloudbased cell counters. These updates are efficiently made available to all users at the same time.
Accurate Three-second Cell Counts
The Corning® Cell Counter can perform a single cell count in less than three seconds*. This is much faster than most automated cell-counting systems. With traditional systems, the image analysis algorithms must be processed on a relatively small onboard computer. The Corning® Cell Counter utilizes the CytoSMART™ App by processing the captured images in the Microsoft Azure Cloud Computing Platform. This cloud computing ability means that it can
analyze the images faster than any existing onboard processer can.
Higher Accuracy
The Corning® Cell Counter uses a cloud-based machine learning algorithm that manages thousands of parameters to provide accuracy without the need to define mammalian cell types. When Trypan Blue is added (Figure 1) the system can also detect cell viability. The Corning® Cell Counter can resolve clusters of cells, which leads to accurate cell counts of “highly concentrated samples” (up to 1 x 107 cells/mL). The new multicount feature allows multiple images per sample thus increasing overall accuracy and reproducibility.
Improved Resolution
New algorithm allows resolution of mammalian cells as small as 4 µm without an equipment upgrade. For viability, the range is 5 to 70 µm and can accurately count PBMC and CAR-T cells.
Low Cost Like Manual Counting
This cell counter works with the provided counting chamber or a customer supplied hemocytometer with a 0.1 mm chamber height, enabling users to enjoy the benefits of automated cell counting without the cost of disposable slides. However, for high throughput needs, most major brands of disposable counting chambers are compatible with the Corning® Cell Counter.
Easy to Use
The Corning® Cell Counter is easy to use. Simply connect the cell counter to your computer or tablet and start the CytoSMART App on your desktop. Place the loaded counting chamber on the stage. Focus on your cells and press the Count button. The simplicity of the
cell counter allows anyone working in your lab to easily count cells without the need of extensive training.
Accessible Data Anywhere, Anytime
With the Corning® Cell Counter, the report is instantly shown on your computer and sent to the CytoSMART Cloud, enabling you to look up the analyzed image and cell count on your smartphone, tablet, or computer. Since all data is saved in the CytoSMART Cloud, you can gain insight into the health and quality of your cell culture from one experiment to the next.
Specifications
Counting range 5 x 104 to 1 x 107 cells/mL
Counting range 4 to 70 µm; 5 to 70 µm with viability
Measurement time <3 sec.*
Compatibility Reusable and disposable counting chambers
Sample volume 10 µL
Weight 1.0 kg
Multicount capability Maximum of 8
Field of view 2.0 x 1.5 mm or 1.39 x 1.39 mm
Magnification 200X
Image resolution 2048 x 1536
Exported formats PNG
Light source LED
Camera 5 MP CMOS
Unit dimensions 122 x 122 x 125 (L x W x H)
Operating environment 5°C to 40°C, 20% to 95% humidity
Requirements:
- Windows 10 PC or Tablet
- Internet connection
- Mammalian cells
- USB 3.0 port
480202 Gridless Disposable Counting Chamber 50 Slides per Case, for use with the Corning® Cell Counter
Gridless Disposable Counting Chamber slide has 2 chambers with 0.1 mm depth and capacity ca. 10µL each.
25-900-CI Corning® 100 mL Trypan Blue Solution, 0.4% (w/v) in PBS, pH 7.5 ± 0.5
Trypan Blue Solution 0.4% (w/v) in normal saline (8.1 g/L NaCl with 0.6 g/L K2HPO4)
Technical Advantage: Can be used for a variety cell counting methods requiring information on cell count and viability.
Trypan Blue is the most common stain used in cell count and viability assays. Viable cells exclude the dye, while nonviable cells absorb the dye and appear blue.
Cells should be in suspension as single cells in buffered saline before counting.
Trypan blue can be used in assays providing total cell count, viable cell or nonviable cell count alone.
This solution is provided at the common concentration of 0.4% (w/v).
Complete Certificate of Analysis available for each production lot, along with full formulation information.
Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.
This product is non-returnable. If you have any questions, please contact us immediately.
Ordering Information
Cat. No. | Description | Qty/Cs |
6749 | Corning® Cell Counter | 1 |
480202 | Gridless Disposable Counting Chamber | 1 |
25-900-CI | Trypan Blue Solution 0.4%, 100 mL | 1 |
Contact EPRO , your local Corning Account Manager to request a demonstration of the Corning Cell Counter.
*Measured using a 73 Mbps download speed and a 20 Mbps upload speed. Actual speed can vary depending on the internet connection.