Buffer solutions

BES, Free acid BES201 CAS number: [10191-18-1] [N,N-Bis(2-Hydroxyethyl)-2-Amino ethanesulfonic Acid] Useful pH: 6.4 - 7.8 pKa (20 °C): 7.1 ± 0.1 C6H15NO5S MW: 213.25 Purity: ≥ 99.0% pH (10%, H2O): 3.5 - 5.0 Solubility (10% water): clear/colorless

BES, Sodium salt BES202 CAS number: [66992-27-61] [N,N-Bis(2-Hydroxyethyl)-2-Amino ethanesulfonic Acid, Sodium salt] C6H14NO5S.Na MW: 235.2 Useful pH: 6.4 - 7.8

BICINE, Biotechnology Grade (N,N-Bis(2-Hydroxyethyl)glycine) CAS number: [150-25-4] Useful pH: 7.6-9.0 pKa: 8.3 C6H13NO4 MW: 163.2 Purity: > 99% Solubility (1 M water): clear/colorless

BIS-TRIS Propane (1,3-Bis[tris(hydroxymethyl)methylamino]propane) CAS number: [64431-96-5] C11H26N2O6 MW: 282.3 Purity: ≥ 98% pKa: 6.8 ± 0.1 Useful pH range: 6.3 - 9.5 Solubility(1M water): clear/colorless

BIS-TRIS, Free Base [Bis (2-Hydroxyethyl)amino-tris(hydroxymethyl)methane] Important buffer for protein and nucleic acid systems. CAS number: [6976-37-0] Purity: ≥ 98% C8H19NO5 MW: 209.2 pKa (25 °C): 6.35 - 6.65 Useful pH range: 5.8 - 7.2

BIS-TRIS, Free Base Molecular Biology Grade, Purity: ≥99% Bis (2-Hydroxyethyl)amino-tris(hydroxymethyl)methane]. Important buffer for protein and nucleic acid systems. Suitable for electrophoresis and cell culture. CAS number: [6976-37-0] C8H19NO5 MW: 209.2 pKa (25 °C): 6.35 - 6.65 Useful pH range: 5.8 - 7.2 Solubility (1M): clear/colorless pH, 1M water: 9.0 - 11.0 A280, 1M water: < 0.40 Heavy metals (as Pb): > 0.0005%

CAPS, Biotechnology Grade [3-(Cyclohexamino)-1-propanesulfonic acid] CAS number: [1135-40-6] C9H19NO3S MW: 221.3 pKa (25 °C): 10.4 Useful pH range: 9.7 - 11.1 Purity: > 99% pH (1% water): 4.9 - 6.0

CHES, Biotechnology Grade CHE702 CAS number: [103-47-9] [2-(N-Cyclohexylamino)ethanesulfonic acid] Useful pH: 8.6 - 10.0 pKa (20 °C): 9.55 C8H17NO3S MW: 207.3 Purity: ≥ 99.0%

HEPES, 1M Sterile Solution HEP003 pH: 7.2 - 7.4 Nuclease activity: none detected

HEPES, Biotechnology Grade HEP001 CAS number: [7365-45-9] C8H18N2O4S MW: 238.31 Purity: ≥ 99.5% pKa (20 °C): 7.5 pH Range: 6.8 - 8.2 Heavy Metals: < 0.001% DNase, RNase, Protease activity: none detected

HEPES, Sodium Salt, Biotechnology Grade HEP002 CAS number: [75277-39-3] C8H17N2O4SNa MW: 260.3 Purity: > 99.5% Solubility (5% water): 7.55 pH (1% water): 9.5 - 11.0 Heavy Metals: < 10 ppm DNase, RNase, Protease activity: none detected

IMIDAZOLE, Biotechnology Grade IMD508 CAS number: [288-32-4] pKa: 6.95 Useful pH: 6.2 - 7.8 C3H4N2 MW: 68.08 Purity: min. 99% Melting Range: 88 - 92 °C Insolubles: < 0.1% DNase, RNase Activity: none detected Color: white to slightly off - white Solubility (0.1 M): clear/colorless

CAS Number: [145224-94-8] [2-(N-Morpholino)ethanesulfonic Acid] C 6 H 13 NO 4 S . H 2 O MW: 213.25 Purity: min. 99.5% pKa(25°C): 6.10 ± 0.05% pH (1%, H2O): 3.5 - 4.1 Heavy metals: max. 0.15 ppm Chloride: max. 0.01% Sulfate: max. 0.05% Solubility (10%, water): clear / colorless Dnase/Rnase/protease activity: none detected

CAS number: [71119-23-8] Ultra Pure Grade [2-(N-Morpholino)ethanesulfonic Acid, Sodium salt] C 6 H 12 NO 4 S.Na MW: 217.24 Purity: min.99.5% Solubility (33%, in water): clear / colorless pH (1% water): 9.0 - 10.5 Heavy metals (as Pb): max. 5 ppm UV Absorbance (33% water) 290 nm: max.0.10 Dnase / Rnase / Protease activity: none detected

pH: 6.8 - 7.2 Dnase and Rnase: none detected Sterile filteredFormulation: 200mM MOPS

CAS number: [71119-22-7] C 7 H 14 NO 4 S . Na MW: 231.25 Purity: min 99.5% pH (0.1M, water): 9.5 - 10.5 Solubility (1M, water): clear / colorless Heavy metals (as Pb): max. 10 ppm A260nm: max. 0.05 A280nm: max. 0.03 Dnase / Rnase / Protease: none detected

CAS Number: [1132-61-2] C 7 H 15 NO 4 S MW: 209.27 Purity: min. 99.5% pH (10%, water): 3.0 - 5.0 Heavy metal (as Pb): max. 10 ppm A260 (1M, water): max. 0.05 Solubility (10% water): clear / colorless Dnase / Rnase activity: none detected

PIPES, Free acid, Biotechnology Grade PIP666 CAS number: [5625-37-6] pKa (20 °C): 6.80 Useful pH range: 6.1-7.5 C8H18N2O6S2 MW: 302.4 Purity: min. 99% DNase/RNase/protease: none detected

PIPES, Sodium Salt (1.5), Biotechnology Grade PIP503 CAS number: [100037-69-2] C8H18N2O6S2 MW: 670.7 Purity: > 99% pKa (20 °C): 6.80 Useful pH range: 6.1-7.5

TAPS, Free Acid TAP001 CAS number: [29915-38-6] C7H17NO6S MW: 243.30 Useful pH range: 7.7 - 9.1 pKa at 25 °C: 8.4 Purity: min. 99.5% Residue upon ignition: > 0.1% Solubility (1 M): clear/colorless UV Abs at 280 nm < 0.01 UV Abs at 260 nm < 0.02 DNase/RNase/Protease: none detected