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Buffer solutions

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Buffer solutions are substances the pH of which does not change when small amounts of strong acids or bases are added to them. Their pH remains unchanged even when the preparation is diluted with water. The success of many reactions depends on the correct concentration of ions used in the solutions – this is why it is important to use precise buffering substances that allow pH regulation.

 

What does the buffer solution consist of? Most commonly it is a mixture of:

 

weak base and its salt with a strong acid,

weak acid and its salt with a strong base,

two acid salts.

 

Taking into account the fact that the role of pH buffers is to maintain a constant reaction of solutions, they are widely used in laboratory work. They are used, among other things, in the production of synthetic drugs and controlling their reaction, but not only. Some are involved in the creation of dyes or polygraphs and others are used to control the pH of finished foods and cosmetics. Buffering substances are also used in fermentation processes.

 

How do pH buffer solutions work?

 

During the conduct of many tests, the analysis of a particular substance is carried out and sometimes the need for an additional preparation arises. This can result in pH fluctuations. The role of buffering substances is to effectively prevent this from occurring and seek to maintain a constant pH of the preparation.

 

Among the most popular pH buffers are buffer solutions:

 

bicarbonate,

acetate,

ammonium,

phosphate,

borate.

 

The precision buffer solutions we offer in our EPRO online shop meet the strict requirements for their composition. This makes it possible to maintain an adequate level of safety and the highest substance quality. This is also important for maintaining the properties and effectiveness of the buffer.

 

How to use buffering agents correctly?

 

Before applying the buffer solution, the parameters of the ingredients (quantity and concentration) must first be calculated – this is determined by the required target volume and application. The ingredients should then be weighed, dissolved and the pH adjusted. The next step will be to make up the solution to the required target volume, describe it and document the actions. The product thus prepared can be stored for future use or used immediately.

 

In the offer of our EPRO online shop, you will find precision pH buffer solutions in various capacities. The products available in this category come from the renowned BioShop® manufacturer for whom top quality and accuracy play the most important role. Our aim is to provide you with products that will guarantee maximum satisfaction during your work. We only offer preparations that have been thoroughly tested before being released. We encourage you to familiarise yourself with our range of products and to place orders.

 

We will provide you with high quality molecular biology products and timely delivery. We also pride ourselves on the repeatability of quality in each successive batch of product, this allows EPRO to build a brand as a reliable partner in working with you.

 

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TAPS, Sodium Salt

TAPS, Sodium Salt

TAP222.100 TAP222.100 | 100 g (TAP222.100), 250 g (TAP222.250), 500 g (TAP222.500)
TAPS, Sodium Salt
TAPS, Sodium Salt, Biotechnology Grade TAP222 CAS number: [91000-53-2] C7H16NO6SNa MW 265.30 Useful pH range: 7.7-9.1 pKa@25 °C: 8.4 Purity: min. 99% DNase/RNase/Protease: none detected
TAPS, Sodium Salt, Biotechnology Grade TAP222 CAS number: [91000-53-2] C7H16NO6SNa MW 265.30 Useful pH range: 7.7-9.1 pKa@25 °C: 8.4 Purity: min. 99% DNase/RNase/Protease: none detected
Package size and cat. number
100 g (TAP222.100), 250 g (TAP222.250), 500 g (TAP222.500)
Manufacturer
BioShop Canada Inc.
Availability
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510,45 zł

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100 g (TAP222.100), 250 g (TAP222.250), 500 g (TAP222.500)
TES, Free Acid

TES, Free Acid

TES001.25 TES001.25 | 25 g (TES001.25), 100 g (TES001.100), 500 g (TES001.500), 1 kg (TES001.1)
TES, Free Acid
TES, Free Acid TES001 CAS number: [7365-44-8] C6H15NO6S MW: 229.2 Purity: > 99% pKa at 25 °C: 7.5 Useful pH range: 6.8 - 8.2 DNase, RNase Protease : none detected
TES, Free Acid TES001 CAS number: [7365-44-8] C6H15NO6S MW: 229.2 Purity: > 99% pKa at 25 °C: 7.5 Useful pH range: 6.8 - 8.2 DNase, RNase Protease : none detected
Package size and cat. number
25 g (TES001.25), 100 g (TES001.100), 500 g (TES001.500), 1 kg (TES001.1)
Manufacturer
BioShop Canada Inc.
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308,73 zł

total
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25 g (TES001.25), 100 g (TES001.100), 500 g (TES001.500), 1 kg (TES001.1)
TRICINE, Ultra Pure

TRICINE, Ultra Pure

TRI001.100 TRI001.100 | 250 g (TRI001.250), 100 g (TRI001.100), 500 g (TRI001.500), 1 kg (TRI001.1)
TRICINE, Ultra Pure
TRICINE, Ultra Pure TRI001 CAS number: [5704-04-1] C6H13NO5 MW 179.2 Purity: > 99% Heavy Metals (as Pb): < 10ppm pKa (25 °C): 8.1 Useful pH range: 7.4-8.8
TRICINE, Ultra Pure TRI001 CAS number: [5704-04-1] C6H13NO5 MW 179.2 Purity: > 99% Heavy Metals (as Pb): < 10ppm pKa (25 °C): 8.1 Useful pH range: 7.4-8.8
Package size and cat. number
250 g (TRI001.250), 100 g (TRI001.100), 500 g (TRI001.500), 1 kg (TRI001.1)
Manufacturer
BioShop Canada Inc.
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371,46 zł

total
Package size and cat. number
250 g (TRI001.250), 100 g (TRI001.100), 500 g (TRI001.500), 1 kg (TRI001.1)
TRIS (Base), Ultra Pure

TRIS (Base), Ultra Pure

TRS001.500 TRS001.500 | 500 g (TRS001.500), 1 kg (TRS001.1), 5 kg (TRS001.5)
TRIS (Base), Ultra Pure
TRIS (Base), Ultra Pure TRS001 CAS number: [77-86-1] C4H11NO3 MW: 121.14 Purity: ≥ 99.9% Insolubles: ≤ 0.005% A290 (1M water.): ≤ 0.05% Heavy Metals (as Pb): ≤ 5 ppm Solubility (1.0M solution): clear/colorless DNase, RNase and Protease activity: none detected
TRIS (Base), Ultra Pure TRS001 CAS number: [77-86-1] C4H11NO3 MW: 121.14 Purity: ≥ 99.9% Insolubles: ≤ 0.005% A290 (1M water.): ≤ 0.05% Heavy Metals (as Pb): ≤ 5 ppm Solubility (1.0M solution): clear/colorless DNase, RNase and Protease activity: none detected
Package size and cat. number
500 g (TRS001.500), 1 kg (TRS001.1), 5 kg (TRS001.5)
Manufacturer
BioShop Canada Inc.
Availability
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611,31 zł

total
Package size and cat. number
500 g (TRS001.500), 1 kg (TRS001.1), 5 kg (TRS001.5)
TRIS ACETATE, Purified

TRIS ACETATE, Purified

TRA222.50 TRA222.50 | 50 g (TRA222.50), 100 g (TRA222.100), 250 g (TRA222.250), 500 g (TRA222.500)
TRIS ACETATE, Purified
TRIS ACETATE, Purified TRA222 CAS number: [6850-28-8] C4H11NO3.C2H4O2 MW: 181.19 Purity: > 98% pH (1% Soln.): 6.5 +/- 0.
TRIS ACETATE, Purified TRA222 CAS number: [6850-28-8] C4H11NO3.C2H4O2 MW: 181.19 Purity: > 98% pH (1% Soln.): 6.5 +/- 0.
Package size and cat. number
50 g (TRA222.50), 100 g (TRA222.100), 250 g (TRA222.250), 500 g (TRA222.500)
Manufacturer
BioShop Canada Inc.
Availability
In Stock In Stock
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423,12 zł

total
Package size and cat. number
50 g (TRA222.50), 100 g (TRA222.100), 250 g (TRA222.250), 500 g (TRA222.500)
TRIS HCl, Ultra Pure

TRIS HCl, Ultra Pure

TRS002.500 TRS002.500 | 500 g (TRS002.500), 1 kg (TRS002.1), 5 kg (TRS002.5)
TRIS HCl, Ultra Pure
TRIS HYDROCHLORIDE, Ultra Pure TRS002 CAS number: [1185-53-1] C4H11NO3. HCl MW: 157.64 Purity: ≥ 99.0% Residue on Ignition: < 0.1% pH (1.0M, H2O): 3.7-4.7 Heavy metals (as Pb): < 5 ppm Solubility (1.0 M aq. Soln.): clear/colorless UV Abs @ 260nm (1.0M): < 0.02 UV Abs @ 280nm (1.0M): < 0.02
TRIS HYDROCHLORIDE, Ultra Pure TRS002 CAS number: [1185-53-1] C4H11NO3. HCl MW: 157.64 Purity: ≥ 99.0% Residue on Ignition: < 0.1% pH (1.0M, H2O): 3.7-4.7 Heavy metals (as Pb): < 5 ppm Solubility (1.0 M aq. Soln.): clear/colorless UV Abs @ 260nm (1.0M): < 0.02 UV Abs @ 280nm (1.0M): < 0.02
Package size and cat. number
500 g (TRS002.500), 1 kg (TRS002.1), 5 kg (TRS002.5)
Manufacturer
BioShop Canada Inc.
Availability
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1 039,35 zł

total
Package size and cat. number
500 g (TRS002.500), 1 kg (TRS002.1), 5 kg (TRS002.5)
TRIS, 1.0M, pH 7.5

TRIS, 1.0M, pH 7.5

TRS111.500
TRIS, 1.0M, pH 7.5
TRIS, 1.0M, pH 7.5 TRS111 CAS number: [77-86-1] C4H11NO3 MW: 121.14 Purity: ≥ 99.9% Insolubles: ≤ 0.005% A290 (1M water.): ≤ 0.05% Heavy Metals (as Pb): ≤ 5 ppm Solubility (1.0M solution): clear/colorless DNase, RNase and Protease activity: none detected Auotclave Sterilized
TRIS, 1.0M, pH 7.5 TRS111 CAS number: [77-86-1] C4H11NO3 MW: 121.14 Purity: ≥ 99.9% Insolubles: ≤ 0.005% A290 (1M water.): ≤ 0.05% Heavy Metals (as Pb): ≤ 5 ppm Solubility (1.0M solution): clear/colorless DNase, RNase and Protease activity: none detected Auotclave Sterilized
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BioShop Canada Inc.
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308,73 zł

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308,73 zł

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TRIS, 1.0M, pH 8.0

TRIS, 1.0M, pH 8.0

TRS222.500
TRIS, 1.0M, pH 8.0
TRIS, 1.0M, pH 8.0 TRS222 CAS number: [77-86-1] C4H11NO3 MW: 121.14 Purity: ≥ 99.9% Insolubles: ≤ 0.005% A290 (1M water.): ≤0 .05% Heavy Metals (as Pb): ≤ 5 ppm Solubility (1.0M solution): clear/colorless DNase, RNase and Protease activity: none detected Auotclave Sterilized
TRIS, 1.0M, pH 8.0 TRS222 CAS number: [77-86-1] C4H11NO3 MW: 121.14 Purity: ≥ 99.9% Insolubles: ≤ 0.005% A290 (1M water.): ≤0 .05% Heavy Metals (as Pb): ≤ 5 ppm Solubility (1.0M solution): clear/colorless DNase, RNase and Protease activity: none detected Auotclave Sterilized
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BioShop Canada Inc.
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308,73 zł

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308,73 zł

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TRIS, 1.0M, pH 8.5

TRIS, 1.0M, pH 8.5

TRS333.500
TRIS, 1.0M, pH 8.5
TRIS, 1.0M, pH 8.5 TRS333 CAS number: [77-86-1] C4H11NO3 MW: 121.14 Purity: ≥ 99.9% Insolubles: ≤ 0.005% A290 (1M water.): ≤ 0.05% Heavy Metals (as Pb): ≤ 5 ppm Solubility (1.0M solution): clear/colorless DNase, RNase and Protease activity: none detected Auotclave Sterilized
TRIS, 1.0M, pH 8.5 TRS333 CAS number: [77-86-1] C4H11NO3 MW: 121.14 Purity: ≥ 99.9% Insolubles: ≤ 0.005% A290 (1M water.): ≤ 0.05% Heavy Metals (as Pb): ≤ 5 ppm Solubility (1.0M solution): clear/colorless DNase, RNase and Protease activity: none detected Auotclave Sterilized
Manufacturer
BioShop Canada Inc.
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308,73 zł

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Total (total)

308,73 zł

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