ALLin™ Mega HiFi Mastermix
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Manufacturer
Product Description
Derived from our HiFi polymerase, the highQu ALLin™ Mega HiFi DNA Polymerase provides much lower error
rate PCR with a 100 higher fidelity compared to Taq. The ALLin™ Mega HiFi DNA Polymerase is engineered
to be much faster and to generate a higher yield of long PCR products up to 20 kb from complex GC-rich templates.
Therefore the ALLin™ Mega HiFi DNA Polymerase is an excellent choice for longer and very complex PCR applications
where the highest fidelity is demanded. It is an enzyme of choice for cloning and all kind of sequencing applications
including NGS. Generated blunt-ended PCR products are suitable for ligation into blunt vectors.
For maximum convenience, the ALLin™ Mega HiFi Mastermix, 2X (HLM0201)
and ALLin™ Mega HiFi Red Mastermix, 2X (HLM0301) are available.
Applications
Fast high-fidelity PCR (up to 100 x Taq).
Long PCR up to 20 kb.
Amplification of complex (GC/AT-rich) templates.
Sequencing, including NGS.
Blunt-end cloning and other applications.
Benefits
Fast, high yield PCR with the fidelity 100x higher than Taq.
Up to 20 kb long PCR even from complex templates.
Increased processivity for faster amplification and higher yield.
High thermostability for better denaturation of GC rich templates.
Best choice for NGS and other sequencing applications.