Ready-to-use Take5™ 1 kb DNA Ladder for approximate DNA size estimation on agarose gels

Opis towaru

highQu ready to use DNA ladders are mixtures of highly purified plasmid digests and PCR products.

The high ladder purity allows for exceptional stability and room temperature storage.

Ladders are ready to be directly loaded on agarose gels and are supplied with the loading dye solution for probe DNA.

They provide sharp bands for DNA sizing and allow for approximate DNA quantification by comparing the amount of DNA

of the band similar in size to your sample band.

The most important factors influencing agarose gel electrophoresis results:

Use the right percentage of the agarose gel for the given size of the DNA to be analysed.

Prepare the gel according to the instructions of the agarose manufacturer.

Use the most sensitive nucleic acid stain (StainIN™ Green) in both agarose gel and in the buffer.

Use the same loading dye both for the samples and for the DNA ladder.

Select DNA ladder which is supplied with the loading dye solution for the DNA samples (Take5™ 1 kb DNA Ladder).

For more precise sizing of the DNA, load the DNA ladder into the two slots of the agarose gel Use enough

of the electrophoresis buffer to keep the agarose gel well-covered during the electrophoresis run.

Visualize the agarose gel to compare your sample bands with the ones of the DNA ladder using an optimal gel documentation system.

Applications

DNA sizing on agarose gels.

Approximate DNA quantification on agarose gels.

Benefits

Room-temperature-stable ladders - always ready to use.

Sharp bands, bright reference bands, indicated DNA mass.

Supplied with loading dye for DNA samples.