EZ-10 Spin Column Fungal RNA Mini-Preps Kit
The kit is designed for preparation of high quality total RNA from a wide range of fungal species.
Fungal samples are lysed and homogenized by Buffer Rlysis-FG.
RNA in the whole homogeneity is selectively absorbed on spin column and other impurities are washed away.
Total RNA is eluted from the membrane in the presence of RNase-Free Water in the final step.
3-5 μg total RNA can be purified from 30 mg filamentous fungi using this kit.
Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly A+ purification, nuclease protection and in vitro translation.