EZ-10 96-Well Plate Blood Genomic DNA Mini-Preps Kit
96-well Plate blood genomic DNA minipreps kit is designed for rapid and high-throughout purification of genomic DNA from fresh or frozen anticoagulated blood. Smaples are first lysed using proteinase K in an optimized buffer. The lysate using proteinase K in an optimized buffer. The lysate is then loaded onto the 96-well EZ plate, DNA is selectively bound to the EZ membrane embed in the plate in the presence of high concentrations of chaotropic salt. During wash steps, protein and other impurities are removed and DNA is then eluted in water or low-salt buffer. Purified DNAA typically has an A260/A280 ratios of 1.7-1.9, and is highly suited for most downstream applications such as PCR, Southern blotting, RAPD and RFLP. The purification procedure requires no phenol or chloroform extraction of alcohol precipitation, and involves minimal handling. The whole procedure takes only 20 minutes after sample preparation.