The ALLin™ Hot Start Bst Polymerase is a recombinant protein, representing a large fragment of the B. stearothermophilus DNA Polymerase expressed in E. coli cells. The activity of the enzyme is blocked at ambient temperature due to molecular inhibition based hot-start technology, and the polymerase is activated only at 45-50°C what reduces non-specific amplification, primer dimer formation and is therefore excellent for ambient reaction setup. This robust polymerase with a strong strand displace

ALLin™ HS Isothermal DNA Amplification Kit enables detection of as little as 3 target DNA molecules in a short time of 20 minutes even without the use of a thermal cycler. The Kit includes a 2X master mix with optimized high-performance buffer, dNTPs and a recombinant Hot Start Bst Polymerase having strong 5’ - 3’ strand displacement activity and efficient 5’ - 3’ polymerase activity working at 55 - 70°C. The activity of the enzyme is blocked at ambient temperature due to molecular inhibition ba

ALLin™ HS Iso-Colorimetric DNA Amplification Kit enables sensitive target DNA molecules detection in real time without the use of any equipment. The kit employs the robust Hot Start Bst polymerase in a master mix with all reaction components and the pH-sensitive inert dye which changes the colour from pale orange to bright yellow as soon as the reaction pH changes with the synthesis of DNA reaching a plateau. The mix detects as little as 3 target DNA molecules in a short time of 20 minutes witho

highQu 1Step RT qPCR master mixes in combination with a blend of thermostable and extremely active Reverse Transcriptase & advanced RNase Inhibitor (RT Mix) allow for a single step one tube RT qPCR. qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT/GC rich templates and guaranty rapid extension with early Ct va

highQu ORA™ qPCR Probe Mixes and 1Step RT qPCR Probe Kits are available in 3 versions – without ROX, with low or high ROX concentration. ROX is a passive reference dye for qPCR, which is used to normalize data in quantitative real-time PCR (qPCR) experiments. The ROX for qPCR Mixes, 50 μM is a great choice for those users who wish to have the universal Probe mix, that can be used either without ROX, or with different ROX concentrations, what allows the applications on all kind of available real