highQu ORA™ qPCR Probe Mixes and 1Step RT qPCR Probe Kits are available in 3 versions – without ROX, with low or high ROX concentration. ROX is a passive reference dye for qPCR, which is used to normalize data in quantitative real-time PCR (qPCR) experiments. The ROX for qPCR Mixes, 50 μM is a great choice for those users who wish to have the universal Probe mix, that can be used either without ROX, or with different ROX concentrations, what allows the applications on all kind of available real

SampleIN™ 1Step RT qPCR Probe Mix has been specifically designed for use with crude lysates and impure templates. This 4X concentrated single-tube RT qPCR master mix with the Hot Start Taq DNA Polymerase, Reverse Transcriptase, RNase inhibitor, dNTPs, magnesium and optimized buffer delivers an exceptional PCR inhibitor tolerance in direct one-step qPCR applications. It includes PCR enhancers and stabilizers and is formulated to provide the robust performance with such common sample materials lik

SampleIN™ 1Step RT qPCR Probe ROX L Mix has been specifically designed for use with crude lysates and impure templates. This 4X concentrated single-tube RT qPCR master mix with the Hot Start Taq DNA Polymerase, Reverse Transcriptase, RNase inhibitor, dNTPs, magnesium and optimized buffer delivers an exceptional PCR inhibitor tolerance in direct one-step qPCR applications. It includes PCR enhancers and stabilizers and is formulated to provide the robust performance with such common sample materia

The SampleIN™ Direct qPCR Probe Mix has been specifically designed for use with unpurified samples or crude lysates as templates. This 4X concentrated qPCR master mix with the Hot Start Taq DNA Polymerase, dNTPs, magnesium, and optimized buffer delivers an exceptional PCR inhibitor tolerance in direct qPCR applications. It includes PCR enhancers and stabilizers and is formulated to provide the robust performance with such common sample materials like unpurified saliva or fresh blood. The mix tol

SampleIN™ Direct qPCR Probe ROX L Mix has been specifically designed for use with unpurified samples or crude lysates as templates. This 4X concentrated qPCR master mix with the Hot Start Taq DNA Polymerase, dNTPs, magnesium, and optimized buffer delivers an exceptional PCR inhibitor tolerance in direct qPCR applications. It includes PCR enhancers and stabilizers and is formulated to provide the robust performance with such common sample materials like unpurified saliva or fresh blood. The mix t

SampleIN™ Lysis Set for PCR/qPCR is a combination of a lysis buffer and protease-containing buffer allowing for a fast lysis of different sample material to release the DNA in a short time. SampleIN™ Lysis Set for PCR/qPCR is a part of our SampleIN™ Direct PCR Kit. SampleIN™ Direct PCR Kit includes two modules: Two Lysis/Protease Buffers (Lysis Set for PCR/qPCR) and Hot Start PCR Master Mix. The quickly lysed samples contain enough DNA for PCR and qPCR/RT-qPCR applications, eliminating the need

SecurRIN™ Advanced RNase Inhibitor is a premium tool for RNA protection from degradation during enzymatic reactions, storage or extraction. It is an extraordinary stable and robust enzyme: it works at up to 60°C temperature, remains active after weeks of room temperature exposure and multiple freezing thawing cycles. It is active in different buffer conditions within a broad pH range of 5.5 to 9.0, and within 0.5 - 1 mM concentration of DTT. SecurRIN™ Advanced RNase Inhibitor is a 50 kDa non-cov

highQu dNTP sets and mixes meet all highest industry standards and allow for unrivaled performance of your PCR and other DNA synthesis, labeling or sequencing reactions. Produced under the stringent quality monitoring conditions, they guaranty reproducible results. More than 99% HPLC purity eliminates inhibitions of PCR and allows for increased yields with higher dNTP concentrations. Exceptional stability of dNTPs allows for short term ambient temperature shipments, short term room temperature s

StainIN™ eco-RED Nucleic Acid Stain, a 10000X concentrated aqueous solution is a significantly safer alternative to ethidium bromide. It is same easy to use, twice as sensitive and much more secure. At least twice as economical as competing products, this novel DMSO-free stain can be also used and disposed with less environmental and health concerns compared to ethidium bromide. StainIN™ eco-RED is a fluorescent dye that allows detection of >0,1 ng of DNA in both agarose and polyacrylamide gels.

highQu qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates and guaranty rapid extension with early Ct values with minimum or no optimization. Our master mixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the ORA™ qPCR Green Master mixes are

High Resolution Melting analysis (HRM) is a fast and simple technique for identification of DNA sequence variations. It allows identifying single nucleotide differences by detecting minor changes in qPCR melting curves. highQu ORA™ HRM qPCR Mix includes a proprietary intercalating saturating dye showing no inhibition for PCR. The dye has the same affinity for both AT or GC rich sequences what leads to highest accuracy in genotyping. The hot-start function in the mix is based on the small molecul

highQu qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates, in multiplexing and guaranty rapid extension with early Ct values with minimum or no optimization. Our master mixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the ORA™ qPCR Probe

ighQu qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates and guaranty rapid extension with early Ct values with minimum or no optimization. ORA™ SEE qPCR mixes provide an additional advantage of a simplified tracking of the process, as they are colored with an inert blue dye to make samples

highQu qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates and guaranty rapid extension with early Ct values with minimum or no optimization. ORA™ SEE qPCR mixes provide an additional advantage of a simplified tracking of the process, as they are colored with an inert blue dye to make sample

highQu qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates, in multiplexing and guaranty rapid extension with early Ct values with minimum or no optimization. ORA™ SEE qPCR mixes provide an additional advantage of a simplified tracking of the process, as they are colored with an inert blue d

highQu PCR Water is a supplementary high quality reagent for all demanding PCR and qPCR and other molecular biology applications. It saves time being on your bench and guaranties the purity of reactions and inhibition-free performance of PCR reagents. highQu PCR Water is a deionized, membrane filtered water continuously tested in ultrasensitive qPCR and PCR applications, in amplification of long targets and highly specific detection of few copies of templates. Applications PCR and qPCR. Other mo

highQu PCRbeam™ Fast PCR Detection Kit is a convenient tool for fast detection of gene-specific amplification products obtained by PCR, LAMP or RPA. The detection is based on immunological reaction driven by Biotin and FITC (fluorescein isothiocyanate), thus the amplified DNA shall include Biotin and FITC labels. The PCR amplification has to be performed with one primer labeled with FITC at 5’-end and one primer labeled with Biotin at 5’-end. Alternatively the use of one of the labeled primers c

Recombinant phi29 DNA Polymerase is a classical enzyme dedicated for use in common isothermal DNA amplification applications that are carried out at moderate temperature based on a strand displacement activity. The enzyme is supplied with an optimized high-performance buffer. The user has to add dNTPs, template and primers. phi29 DNA Polymerase was discovered and characterized by M. Salas. The Polymerase has strong strand displacement activity and efficient 5’-3’ polymerase activity working at a

Proteinase K (Molecular Biology Grade Solution) is a serine peptidase with a very high specific activity and a broad spectrum of protein digestion possibilities. The solution is designed to be used for protein degradation (up to tetrapeptides) during the cell lysis and RNA/DNA extraction procedures under hush reaction conditions such as the higher temperatures and the presence of detergents. The enzyme efficiently degrades DNases and RNases during nucleic acid isolation process. The high purity

highQu ready to use DNA ladders are mixtures of highly purified plasmid digests and PCR products. The high ladder purity allows for exceptional stability and room temperature storage. Ladders are ready to be directly loaded on agarose gels and are supplied with the loading dye solution for probe DNA. They provide sharp bands for DNA sizing and allow for approximate DNA quantification by comparing the amount of DNA of the band similar in size to your sample band. The most important factors influe