4°C CAS number: [30827-99-7] C 8 H 10 FNO 2 S.HCl MW: 239.7 High water soluble alternative to PMSF. Less toxic than PMSF. Well suited in large scale purification of recombinant proteins. Irreversibly inactivates thrombin and other serine proteases. Purity: > 97% Solubility (water): 10 g/l Stock solutions: 20-100 mM in water or buffer Solutions have an acidic pH Stability: > 2 months at +4°C As pH increases above neutral stability decreases Stock solutions: stored at –20°C stability apprx. 6 mont

CAS number: [100938-10-1] - 20°C C 21 H 38 N 4 O 8 .HCl MW: 511.00

ANTIPAIN, Dihydrochloride ANT604 General serine and cysteine protease inhibitor. Inhibits trypsin, papain, cathepsin A. CAS number: [37682-72-7] -20 °C C27H44N10O6.2HCl MW: 677.63 Solubility: Water: 50mg/ml DMSO: soluble Methanol: soluble Working concentration: 10-50 μg/ml Typical stock solutions can be prepared in water, DMSO, or methanol at concentrations of 10mg/ml.

APROTININ, Bovine Lung, Lyophilized Powder APR600 CAS number: [9087-70-1] 4 ° C Suitable for Tissue Culture Aprotinin is a broad spectrum protese inhibitor. Inhibits trypsin, chymotrypsin, kallikrein from different sources and plasmin. Activity: > 6000KIU/mg or > 4.0 IU/mg 1 Inhibitor Unit (IU) = 1500 Kallikrein Inhibitor Unit (KIU) Purity: Single band @ 6.5KDa Endotoxin: < 5.0 EU/mg Mycoplasma: none detected Solubility: water, 10mg/ml. Also soluble in 70% methanol and 7

BENZAMIDINE HCL, Ultra Pure (Hydrate) 4 °C CAS number: [1670-14-0] C7H8N2.HCl MW: 156.61 Purity: min. 98% Solubility (1% water): clear/colorless

BESTATIN Inhibitor for Aminopeptidase B and Leucine aminopeptidase. -20 °C Microbial product. CAS number: [58970-76-6] C16H24N2O4 MW: 308.38 Purity: > 98% Solubility: DMSO

CHYMOSTATIN CHY222 CAS number: [9076-44-2] Microbial Product -20 ° C Protease Inhibitor, inhibitor of chymotrypsin, papain and Cathepsin A, B and C Solubility (DMSO): 10 mM Stability: stock soln. >3 months -20°C

E64 EEL640 CAS number: [66701-25-5] Protease Inhibitor. Irriversable inhibitor of several cysteine proteases. Concentrations of 10-30 μM will completely inhibit proteases within 30 minutes. - 20 °C C15H27N5O5 MW: 357.4 Purity: min. 99%

EBELACTONE A EBE001 CAS number: [76808-16-7] Protease Inhibitor. Microbial Source 4 °C C20H34O4 MW: 338.5

Storage at -20°C. CAS number: [103476-89-7] C 20 H 38 N 6 O 4 .0.5H 2 SO 4 MW: 475.6 Purity: min. 95% Appearance: white to off-white powder Solubility: water, methanol or 100% ethanol Synthetic source

PEPSTATIN A, Synthetic Source PEP605 CAS number: [26305-03-3] Aspartic acid protease Inhibitor - 20 °C C34H63N5O9 MW: 685.9 Purity (HPLC): 95% min. Solubility: DMSO, methanol (1mM)

PHENYLMETHYL SULFONYL FLUORIDE (PMSF) PMS123 CAS number: [329-98-6] Inhibits serine and cysteine proteases like trypsin, chymotrypsin, kallikrein and thrombin. C6H5CH2SO4F MW: 174.19 Purity: > 99% Melting Range: 91 - 93 °C

PHOSPHORAMIDON PHS999 CAS number: [119942-99-3] Microbial Source. Inhibitor of collagenase, thermolysin and other meatlloproteases. - 20 °C C23H34N3O10PNa2 MW: 588.5 Purity: min 97% Solubility: methanol, DMSO Working concentration: 5 - 350 μg/ml (7 - 600 μM)

PROTEASE INHIBITOR COCKTAIL, LYOPHILIZED POWDER PIC001 For General Use. Water soluble protease inhibitors Lyophilized and ready to use. 1 Vial makes 1ml of 100x concentrate To prepare stock solution, add 1.0 ml of deionized water and mix gently. To prepare working solution dilute in 100ml of sample buffer. -20° C PIC001: AEBSF, Aprotinin, Bestatin, E64, Leupeptin and EDTA.

Storage at -20°C. Packaged in a ready-to-use lyophilized powder. One vial is recommended for the inhibition of proteases present in 20 g of cell extract. Reconstitute each vial with 100 ml of deionized water to obtain a 100 ml cocktail solution. COMPONENTS AND THEIR CONCENTRATIONS: AEBSF HCl: 2 mM Aprotinin: 0.3 µM Bestatin HCl: 130 µM E64: 14 µM Leupeptin: 1.0 µM

Protease Inhibitor Cocktail, Mammalian, LYOPHILIZED POWDER PIC003 Reconstitute to 1ml with deionized water to form a 100X solution. -20 °C 1ml (100X) solution contains: AEBSF.HCl: 100mM Aprotinin (recombinant): 80uM Bestatin: 5mM E-64: 1.5mM Leupeptin: 2 mM Pepstatin A: 1mM Recommended for use with mammalian cell and tissue extracts. Animal - Free.

TRYPSIN INHIBITOR, Soybean TIS571 4 °C Purified, Lyophilized powder Chromatographically purified. A dialyzed lyophilized powder one mg will inhibit >1.2 mg of trypsin with activity of approximately 12,400 BAEE units/mg of protein.

TRYPSIN INHIBITOR, Soybean TIS587 Partially purified 4° C