Is a universal reagent for processing biological samples for direct PCR.
No isolation of DNA is required. The DNAzol® Direct procedure is simple.
Lyse a sample in DNAzol® Direct for 15 minutes, add an aliquot of the resulting lysate to a PCR mix,
and perform amplification of a selected DNA fragment(s).
The standard DNAzol® Direct procedure supports PCR amplification of DNA fragments up to 8 kb long.
DNAzol® Direct (U.S. patent 7,727,718) composition and procedure are based on the use of an alkaline solution
containing polyethylene glycol and other additives. DNAzol® Direct quickly and effectively lyses biological samples,
releasing DNA into the lysate. The combined effects of the alkaline pH and chaotropic properties of DNAzol® Direct sufficiently
inactivate PCR inhibitors including proteases and nucleic acid degradation enzymes. After processing a sample in DNAzol® Direct,
DNA is denatured into a single-stranded form, RNA is hydrolyzed, and proteins are denatured and partially hydrolyzed.
Due to its unique composition, the DNAzol® Direct lysate does not require neutralization before its use in PCR. The resulting pH
of a PCR mix containing less than 10% of the lysate is within the effective range for PCR.
DNAzol® Direct is designed to process a wide range of samples including animal, plant, fungi, yeast, bacterial and viral
samples. Specific examples are of animal - derived samples: liver, lung, spleen, brain, blood, serum, saliva, hairs and feathers;
plant - derived samples: leaves, tissue fragments and seeds; and samples containing yeast, fungi, bacteria and viruses.